rabbit polyclonal antibody against drd2  (Millipore)

Journal: PLoS ONE

Article Title: The Effect of Chronic Antipsychotic Drug on Hypothalamic Expression of Neural Nitric Oxide Synthase and Dopamine D2 Receptor in the Male Rat

doi: 10.1371/journal.pone.0033247

Figure Lengend Snippet: Haloperidol and risperidone increased DRD2 mRNA expression in both the MPOA and the PVN. Data were relative to β-actin mRNA. * p <0.05 vs Vehicle.

Article Snippet: The membranes were treated with a blocking buffer (5% nonfat dry milk with 0.1% Tween-20) and incubated with primary nNOS and DRD2 antibody respectively (mouse monoclonal antibody against nNOS 1∶500, Transduction Laboratories, Lexington, KY; Rabbit polyclonal antibody against DRD2 1:200, Chemicon International Inc., Temecula, CA) at 4°C overnight.

Techniques: Expressing

Journal: PLoS ONE

Article Title: The Effect of Chronic Antipsychotic Drug on Hypothalamic Expression of Neural Nitric Oxide Synthase and Dopamine D2 Receptor in the Male Rat

doi: 10.1371/journal.pone.0033247

Figure Lengend Snippet: The calculated ODs of nNOS (B) and DRD2 (D) protein bands were normalized to each β-tubulin OD and expressed as a ratio. nNOS protien in the MPOA but not the PVN was significantly decreased by haloperidol. Haloperidol and risperidone increased DRD2 protien expression in both the MPOA and the PVN. Lanes: 1 = Vehicle; 2 = haloperidol; 3 = risperdone; 4 = quetiapine. Data are means ± s.e.means and expressed in arbitrary units. * p <0.05 vs Vehicle; ** p <0.001 vs Vehicle.

Article Snippet: The membranes were treated with a blocking buffer (5% nonfat dry milk with 0.1% Tween-20) and incubated with primary nNOS and DRD2 antibody respectively (mouse monoclonal antibody against nNOS 1∶500, Transduction Laboratories, Lexington, KY; Rabbit polyclonal antibody against DRD2 1:200, Chemicon International Inc., Temecula, CA) at 4°C overnight.

Techniques: Expressing